This project will broaden my intellectual perspectives within the expansive field of neuroscience. My professional life, at the single-molecule end of the neuroscience spectrum, has focused on ion channel proteins. This new direction project would take me toward understanding a complex multi-cellular process - the regeneration of nerve cells and their connections after injury. This new direction would include learning new techniques in computational biology, advanced microscopy, and animal behavior. I propose a summer research collaboration with Dr. Jennifer Morgan at the Marine Biological Laboratories (MBL) in Woods Hole, Massachusetts. Dr. Morgan applies techniques in cell biology, microscopy, and computational biology to understand the processes that allow lamprey spinal cord neurons to survive, grow, and regenerate. The MBL is a world-class scientific community that vibrates with scientific energy every summer. I propose to study changes in expression of ion channels and receptors in lamprey spinal cord following injury. Expression and localization will be studied by immunofluorescence microscopy. I will participate in a Genome Regulatory Network approach to study the changes in gene transcription using an existing dataset. This project will provide professional development in a new area of neurobiology. The pilot study at the MBL would be renewing because the scientific energy and joy of discovery there is contagious. The long-term impact will be to expand the portfolio of experimental questions I can offer to Earlham students.