The proposed project is designed to develop methods to assess how amphiphilic peptides cause cell lysis or cell death. Over 50 years ago, amphiphilic peptides, or host defense peptides, were discovered in every form of life and were referred to as 'Nature’s antibiotics'. These natural antibiotics, however, have not found extensive use in vivo because of their inherent toxicity. Several modes of action have been proposed, but exactly how these peptides exert their actions is not known. I have developed a potential way to study how natural amphiphilic peptides work by studying a series of peptides derived from non-antimicrobial proteins I have been studying for over 10 years. The work proposed here require developing new techniques that involve (a) growing and studying peptide treated bacterial cells, and (b) using new instrumentation available at the College. These methods are a departure from the work that has been ongoing in my lab, and are a new and exciting direction in my research. This work will additionally add to my professional growth as it will invigorate my research program, make my research more amenable to undergraduate student work, and help me enhance topics covered in my classrooms.